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Qualitative Analysis of Food Products
The phenolic composition of skin and flesh from Malus domestica apples (Anna cultivar)
and Prunus domestica plums (satsuma cultivar) commercial cultivars in Costa Rica, was studied
using Ultra Performance Liquid Chromatography coupled with High Resolution Mass
Spectrometry (UPLC-DAD-ESI-MS) on enriched-phenolic extracts, with particular emphasis in
proanthocyanidin and flavonoids characterization. A total of 52 compounds were identified,
including 21 proanthocyanidins ([(+)-catechin and (−)-epicatechin]) flavan-3-ols monomers,
five procyanidin B-type dimers and two procyanidin A-type dimers, five procyanidin B-type
trimers and two procyanidin A-type trimers, as well as one procyanidin B-type tetramer,
two procyanidin B-type pentamers, and two flavan-3-ol gallates); 15 flavonoids (kaempferol,
quercetin and naringenin derivatives); nine phenolic acids (protochatechuic, caffeoylquinic,
and hydroxycinnamic acid derivatives); five hydroxychalcones (phloretin and 3-hydroxyphloretin
derivatives); and two isoprenoid glycosides (vomifoliol derivatives). These findings constitute
the first report of such a high number and diversity of compounds in skins of one single plum
cultivar and of the presence of proanthocyanidin pentamers in apple skins. Also, it is the first time
that such a large number of glycosylated flavonoids and proanthocyanidins are reported in skins
and flesh of a single plum cultivar. In addition, total phenolic content (TPC) was measured with
high values observed for all samples, especially for fruits skins with a TPC of 619.6 and 640.3 mg
gallic acid equivalents/g extract respectively for apple and plum. Antioxidant potential using
2,2-diphenyl-1-picrylhidrazyl (DPPH) and oxygen radical absorbance capacity (ORAC) methods
were evaluated, with results showing also high values for all samples, especially again for fruit skins
with IC50 of 4.54 and 5.19 μg/mL (DPPH) and 16.8 and 14.6 mmol TE/g (ORAC) respectively for
apple and plum, indicating the potential value of these extracts. Significant negative correlation was
found for both apple and plum samples between TPC and DPPH antioxidant values, especially for
plum fruits (R = −0.981, p < 0.05) as well as significant positive correlation between TPC and ORAC,
also especially for plum fruits (R = 0.993, p < 0.05) and between both, DPPH and ORAC antioxidant
methods (R = 0.994, p < 0.05).
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